Best way is to plot it on a graph or to use a program like graph pad or link weaver
Enzyme kinetics (experiments measuring Vo as a function of [S] to determine Vmax and Km)
– can use computer with programs to fit data to Michaelis-Menten equation, extracting Km and Vmax from the data.
– can’t extrapolate "by hand" on Michaelis-Menten Vo vs. [S] hyperbolic plot to get accurate Vmax and Km values
Vo = Vmax [S]/(Km + [S])
– simple solution without a computer: use linear version, double reciprocal plot (Lineweaver-Burk Plot)
– Take reciprocal of both sides of M-M Equation and rearrange to get Lineweaver-Burk equation
1/Vo = Km/Vmax 1/[S] + 1/V[max]