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Vmax and Km?

When dealing with Vmax and Km in enzyme kinetics;How do you calcuate them?

Asked anonymously on April 25th 2012 5:14 p.m.

1 Answer to this question...

Answered by Marco Tafarri on April 26th 2012 10:43 a.m.
Best way is to plot it on a graph or to use a program like graph pad or link weaver Enzyme kinetics (experiments measuring Vo as a function of [S] to determine Vmax and Km) – can use computer with programs to fit data to Michaelis-Menten equation, extracting Km and Vmax from the data. – can’t extrapolate "by hand" on Michaelis-Menten Vo vs. [S] hyperbolic plot to get accurate Vmax and Km values Vo = Vmax [S]/(Km + [S]) – simple solution without a computer: use linear version, double reciprocal plot (Lineweaver-Burk Plot) – Take reciprocal of both sides of M-M Equation and rearrange to get Lineweaver-Burk equation 1/Vo = Km/Vmax 1/[S] + 1/V[max]