Are You a Lecturer? Improve your student engagement... Sign up your class
Vmax and Km?
When dealing with Vmax and Km in enzyme kinetics;How do you calcuate them?
1 Answer to this question...
Best way is to plot it on a graph or to use a program like graph pad or link
weaver Enzyme kinetics (experiments measuring Vo as a function of [S] to
determine Vmax and Km) – can use computer with programs to fit data to
Michaelis-Menten equation, extracting Km and Vmax from the data. – can’t
extrapolate "by hand" on Michaelis-Menten Vo vs. [S] hyperbolic plot to get
accurate Vmax and Km values Vo = Vmax [S]/(Km + [S]) – simple solution without
a computer: use linear version, double reciprocal plot (Lineweaver-Burk Plot)
– Take reciprocal of both sides of M-M Equation and rearrange to get
Lineweaver-Burk equation 1/Vo = Km/Vmax 1/[S] + 1/V[max]